Ramp sequences can have a large affect on gene expression. We present extensive analyses of ramp sequences in human and SARS-CoV-2 genes, across tissues and cell types. The Ramp Atlas is a website that enables users to quickly calculate ramp sequences in small fasta files. Users can also download data in convenient CSV files about the prevalence of ramp sequences in genes calculated from stratified tissue and cell types. Enjoy!
This altered initiation of translation increases gene expression by evenly spacing ribosomes and reducing mRNA secondary structure. The graph below shows the translational efficiency (measured as a Relative Synonymous Codon Usage, or RSCU, value) of codons throughout the gene. The orange segment is the ramp sequence which has a mean translational efficiency of 0.4531, while the entire gene has a mean efficiency of 0.7734. A translational efficiency of 1.0 would be the most rapidly translated.